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Vitamin B2, (Riboflavin)
    Close Vitamin B2, (Riboflavin)

    Vitamin B2, (Riboflavin)

    Product Code: LVB2

    Price: $116.00

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    Test Description

    Vitamin B2, (Riboflavin)

    Vitamin B2 is also known as riboflavin. Riboflavin is essential in energy production as FAD. It is important in the synthesis and breakdown of fats. It activates the vitamins B6 and folic acid. It is also important in the synthesis of corticosteriods, red blood cells and glycogen. It is found in Brewer's yeast, liver, meat, broccoli, dairy products, wheat germ, poultry, and whole grain products.

    A deficiency of riboflavin is associated with skin problems especially around the nose, mouth, and ears. In addition, a patient may have a "smooth tongue," redness, burning and excessive tearing of the eyes, light sensitivity, anemia, personality changes and cataracts. The RDA for riboflavin is 1.3 mg. A supplemental level is suggested as 1.7 to 50 mgs. There is no toxic dose listed for riboflavin.

    Specimen is 2.0 mL of washed heparinized RBCs. Collect the sample in a heparin tube. Centrifuge the tube, then wash the RBCs one time with normal saline. Protect from light and ship in amber plastic tube.

    Doctors who wish to order kits, please call our lab directly to set up an account before ordering. 316-684-7784

    Test Details

    Process time: 5 to 7 business days

    Process method: The methodology is a HPLC method modified and developed in our laboratory.

    CPT code: 84252

    Special instructions: None

    Rejection causes: Gross Hemolysis

    Collection Instructions

    Patient prep: None

    Specimen volume: 2 mL Heparin RBC; washed 1x, light protected

    Minimum volume: 1.0 mL

    Collection container: Heparin tube

    Transport container: Amber plastic transport tube

    Transport instructions: Freeze


    Normal values: The normal value established in our laboratory for red blood cell vitamin B2 is 3.5 to 20 ug/L.


    Reference one: Gerrits J, Eidhof JW, Brunnekreeft WI, Hessels J: Determination of thiamin and thiamin phosphates in whole blood by reversed phase liquid chromatography with precolumn derivatization: Methods in enzymology, 1997. 279:74-82.

    Reference two: Smith MD: Rapid method for determination of riboflavin in urine by high performance liquid chromatography. J of Chrom, 1980; 182:285-291.

    Reference three: Tietz Textbook of Clinical Chemistry, 3rd Ed, 1999, Saunders Co.

    Reference four: McCormick, DB.: Coenzymes, Biochemistry Of. In Encylopedia of Molecular, Molecular biology and molecular medicine. R.A. Meyers, Ed., New York, 1996. PP 390-406

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