Test Description
Indican, Urine (Indoxyl Sulfate)
Test Details
Process time: 5 to 7 business days
Process method:
Urine Indican Test (Obermeyer test - Indoxyl Sulfate)
Following absorption, indole is converted to 3-hydroxy indole (indoxyl potassium sulfate and indoxyl glucoronate, or indicans in the liver.
Indole (oxidized) => indoxyl + H2O2 => indoxyl sulfuric acid K + indoxyl potassium sulfate (indican)
CPT code: 84999
Special instructions: None
Rejection causes: Less than 5.0 mL received
Collection Instructions
Patient prep: None
Specimen volume: 12 mL urine
Minimum volume: 5.0 mL
Collection container: Clean container
Transport container: Plastic transport tube
Transport instructions: Freeze. If the assay is not run immediately, place sample in labeled plastic tube and freeze.
Collection instructions: Click here for PDF
Results
Normal values:
Detection of indican in the urine depends upon its decomposition and subsequent oxidation of indoxyl to indigo blue and its absorption into a chloroform layer. The resulting color is visually compared to a color chart and graded as follows:
Negative (Normal) = Clear or blue tinge
1+ (Normal) = Slight blue, yellow, mint green
2+ (Positive) = Dark blue, light green, golden brown
3+ (High Positive) = Violet, indigo, dark brown
4+ (Very High Positive) = Jet black
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