Product Code: LHis
Process time: 5 to 7 business days
Process method: The methodology for histamine is a fluorescent method following an ion-exchange separation technique.
CPT code: 83088
Special instructions: None
Rejection causes: Inadequate specimen added to TCA aliquot; specimen not frozen.
Patient prep: Discontinue antihistamines two days before collection of specimen
Specimen volume: Draw until blood stops flowing into tube provided by Bio Center Lab. These tubes will draw blood to just below the black mark on the label. Immediately transfer unclotted blood into 5 mL 10% trichloroacetic acid (TCA). Mix specimen well by vigorously shaking TCA tube after adding blood. Stable one month if kept frozen. Note: Histamine, Spermidine & Spermine testing may all three be performed from a single specimen (i.e. separate aliquots are not required).
Minimum volume: same
Collection container: Non-additive tube; draw this tube last if other tubes are being collected from the patient; do not allow whole blood to clot
Transport container: Plastic transport tube
Transport instructions: Freeze
Collection instructions: Click here for PDF
Normal values: The reference value established in our laboratory for histamine is 33 to 65 ng/mL.
Reference one: Jackson, J.A., Riordan, H.D., Neathery, S., Revard, C. Histamine levels in health and diseases. Jour OrthoMol Med. 1998. 13(4); pp236-240.
Reference two: Pfeiffer, C.C., Smyrl, E.G., Iliev. Extreme basophil counts and blood histamine levels in schizophrenic patients as compared to normals. Res Commun Chem Pathol Pharmacol, 1972a; 4:51.
Reference three: Schwartz, J.C. Histamine as a transmitter in brain. Life Sci, 1975; 17: 503-517.
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